Joseph E. Mazurkiewicz, PhD
Areas of Study
- Yale University School of Medicine 1975Postdoctoral Training
- University of Colorado Health Science Center1973PhD
As Director of the AMC Imaging Core Facility, I am responsible for the maintenance and development of the several microscopes in the AMC Imaging Core Facility that includes a Zeiss LSM880 NLO with Airyscan Super Resolution Inverted Scanning Confocal Microscope system. Researchers are trained to become independent users of the microscopes and image acquisition software with an eye to ensure collection of high-quality images.
Dr. Mazurkiewicz is involved in a variety of collaborations that utilize advanced imaging technologies that include confocal laser scanning microscopy (CLSM), 2-photon scanning microscopy, Total Internal Reflection Florescence Microscopy (TIRFM) and Stimulated Emission Depletion (STED) microscopy. In these collaboration several advanced biophysical imaging modalities have been applied that include, FRET and FRAP, bimolecular fluorescence complementation analyses (BIFC), and fluorescence fluctuation spectroscopy methods that include confocal Fluorescence Correlation Spectroscopy (FCS), and Raster Image Correlation Spectroscopy (RICS). In the imaging core facility, we are currently involved in developing Fluorescence Lifetime Imaging-FRET (FLIM-FRET) imaging to visualize and quantitate ligand-receptor (target engagement) in vitro and in vivo and FLIM imaging to assess the metabolic state of cells and tissues using endogenous sources of fluorescence.
Among my current collaborations, two studies are of most recent interest:
- Pathogenesis of Friedreich ataxia (FRDA) an autosomal recessive neurodegenerative disorder that affects children and young adults. CLSM on post-mortem FRDA brain and DRG sections is the prime imaging modality used.
- Oligomerization and trafficking of Class A neurotransmitter G protein-coupled receptors (GPCR) and the gonadotropin GPCR using FCS, PCH and FRET and using FCS, PCH, BIFC and FRET. G protein-coupled receptors (GPCR) are a prominent class of plasma membrane proteins that regulate physiological responses to a wide variety of external stimuli and therapeutic agents. Each GPCR was C-terminally labeled with YFP or GFP or mCherry and expressed on the plasma membrane of HEK293 cells in live cell culture, either singly or in pairs.
While Dr. Mazurkiewicz is conducting research in collaboration with many investigators he is not currently accepting students into his lab.
Miao L, Li J, Li J, Lu Y, Shieh D, Mazurkiewicz JE, Barroso M, Schwarz JJ, Xin HB, Singer HA, Vincent PA, Zhong W, Radice GL, Wan LQ, Fan ZC, Huang G, Wu M. Cardiomyocyte orientation modulated by the Numb family proteins-N-cadherin axis is essential for ventricular wall morphogenesis. Proc Natl Acad Sci U S A. 2019 Jul 12;. doi: 10.1073/pnas.1904684116. [Epub ahead of print].
Friedreich Ataxia: Developmental Failure of the Dorsal Root Entry Zone. Koeppen AH, Becker AB, Qian J, Gelman BB, Mazurkiewicz JE. Journal of neuropathology and experimental neurology. 2017; 6(11):969-977.
Beta2-adrenergic receptor homodimers: Role of transmembrane domain 1 and helix 8 in dimerization and cell surface expression. Parmar VK, Grinde E, Mazurkiewicz JE, Herrick-Davis K. Biochim Biophys Acta Biomembr. 2017 Sep;1859(9 Pt A):1445-1455. doi: 10.1016/j.bbamem.2016.12.007. Epub 2016 Dec 18.
Koeppen AH, Ramirez RL, Becker AB, Mazurkiewicz JE. Dorsal root ganglia in Friedreich ataxia: satellite cell proliferation and inflammation. Acta Neuropathologica Communications. Neuroscience of Disease 2016; 4(1):46.
Pathology of Intercalated Discs in Friedreich Cardiomyopathy. Ramirez RL, Becker AB, Mazurkiewicz JE, Feustel PJ, Gelman BB, Koeppen AH. J Am Coll Cardiol. 2015 Oct 13;66(15):1739-40. doi: 10.1016/j.jacc.2015.06.1355.
View Joseph E. Mazurkiewicz's articles on the National Institute of Health's PubMed website.