John J. Schwarz, PhD
Areas of Study
MEF2 transcription factors
- University of Texas1988Graduate School of Biomedical SciencesPhD
Our research is directed at understanding how transcriptional regulation in endothelial cells controls activities of the endothelium such as modulating inflammation, thrombosis, and smooth muscle functions. The focus has been on MEF2 transcription factors. Their ability to activate transcription is increased by conditions that protect against atherosclerosis such as laminar shear stress and statin treatment. As such they are good candidates to understand how the endothelium protects against atherosclerosis and how transcriptional regulation can be used directly as a therapeutic target or indirectly to identify downstream targets that are more amenable for intervention. We found that one member of this family, Mef2c, in the endothelium inhibits smooth muscle migration into the intima, which is an important component of several vascular diseases. Specifically, endothelial-specific deletion of Mef2c causes migration of smooth muscle cells through fenestrations in the internal elastic lamina into the intima subjacent to the endothelium. We are currently investigating the mechanism through which Mef2c regulates either a repulsive or attractive signal from the endothelium to medial smooth muscle.
Genetics. 1989 Apr;121(4):635-49. doi: 10.1093/genetics/121.4.635. PubMed PMID: 2566556; PubMed Central PMCID: PMC1203649.The isolation and sequence of missense and nonsense mutations in the cloned bacteriophage P22 tailspike protein gene.
J Biol Chem. 1989 Nov 25;264(33):20112-9. PubMed PMID: 2531143.Characterization of bacteriophage P22 tailspike mutant proteins with altered endorhamnosidase and capsid assembly activities.
Genetics. 1990 Aug;125(4):673-81. doi: 10.1093/genetics/125.4.673. PubMed PMID: 2144496; PubMed Central PMCID: PMC1204093.Intragenic suppression of a capsid assembly-defective P22 tailspike mutation.
Plant Mol Biol. 1990 Dec;15(6):865-77. doi: 10.1007/bf00039426. PubMed PMID: 2103478.The intergenic region of maize streak virus contains a GC-rich element that activates rightward transcription and binds maize nuclear factors.
Mol Cell Biol. 1992 Jan;12(1):266-75. doi: 10.1128/mcb.12.1.266-275.1992. PubMed PMID: 1309591; PubMed Central PMCID: PMC364092.The basic region of myogenin cooperates with two transcription activation domains to induce muscle-specific transcription.
View John J. Schwarz's articles on the National Institute of Health's PubMed website.