University College London,UK
Cambridge University, UK
Our lab is focused on the role of neural progenitor cells in building and maintaining the central nervous system (CNS). Neural progenitor cells include multipotent, self-renewing neural stem cells and other more restricted progenitor cells such as neuroblasts and glioblasts. We study how these different classes of CNS progenitors produce the various types of neuronal and glial progeny, both in vivo and in vitro, focusing primarily on the development of the cerebral cortex. We have developed methods to study the lineage trees of isolated progenitor cells in vitro, following their asymmetric and symmetric division patterns and the types of progeny they produce. Collaborating with Dr. Andrew Cohen at Drexel University, we have helped produce user-friendly software to trace the movements, divisions and lineages of individual cells. In addition to studying isolated cells, we also test the effect of key environmental factors on neural progenitor development, such as factors derived from the vascular niche that is critical for maintaining neural stem cell self-renewal and neurogenesis. By examining the adult neurogenic niche in whole mount preparations, we can assess how neural stem cells interact with the vascular system, and how this changes with disease and aging. Recently we have incorporated studies using human pluripotent stem cells to study both cortical development and retinal development, because using human cells is immediately relevant to disease. An important focus of our lab is translational research, in which lab findings are brought towards the clinic.
- Wait E, Winter M, Bjornsson C, Kokovay E, Wang Y, Goderie S, Temple S, Cohen AR. (2014) Visualization and correction of automated segmentation, tracking and lineaging from 5-D stem cell image sequences. BMC Bioinformatics. Oct 3;15:328. doi: 10.1186/1471-2105-15-328.PMID: 25281197 [PubMed - in process]
- van de Leemput J, Boles NC, Kiehl TR, Corneo B, Lederman P, Menon V, Lee C, Martinez RA, Levi BP, Thompson CL, Yao S, Kaykas A, Temple S, Fasano CA. (2014) CORTECON: a temporal transcriptome analysis of in vitro human cerebral cortex development from human embryonic stem cells. Neuron. 2;83(1):51-68. doi: 10.1016/j.neuron.2014.05.013.PMID: 24991954 [PubMed - indexed for MEDLINE]
- Stanzel BV, Liu Z, Somboonthanakij S, Wongsawad W, Brinken R, Eter N, Corneo B, Holz FG, Temple S, Stern JH, Blenkinsop TA.Human RPE stem cells grown into polarized RPE monolayers on a polyester matrix are maintained after grafting into rabbit subretinal space.Stem Cell Reports. 2014 Jan 2;2(1):64-77. doi: 10.1016/j.stemcr.2013.11.005. eCollection 2014 Jan 14.PMID: 24511471 [PubMed - in process]
- Gage FH, Temple S. Neural stem cells: generating and regenerating the brain.Neuron. 2013 Oct 30;80(3):588-601. doi: 10.1016/j.neuron.2013.10.037. Review.PMID: 24183012 [PubMed - indexed for MEDLINE]
- Kusek G, Campbell M, Doyle F, Tenenbaum SA, Kiebler M, Temple S.Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.Cell Stem Cell. 2012 Oct 5;11(4):505-16. doi: 10.1016/j.stem.2012.06.006. Epub 2012 Aug 16.PMID: 22902295 [PubMed - indexed for MEDLINE] Free PMC Article
- Kokovay E, Wang Y, Kusek G, Wurster R, Lederman P, Lowry N, Shen Q, Temple S. VCAM1 is essential to maintain the structure of the SVZ niche and acts as an environmental sensor to regulate SVZ lineage progression.Cell Stem Cell. 2012 Aug 3;11(2):220-30. doi: 10.1016/j.stem.2012.06.016.PMID: 22862947 [PubMed - indexed for MEDLINE] Free Article
- Aboody K, Capela A, Niazi N, Stern JH, Temple S.Translating stem cell studies to the clinic for CNS repair: current state of the art and the need for a Rosetta stone.Neuron. 2011 May 26;70(4):597-613. doi: 10.1016/j.neuron.2011.05.007. Review.PMID: 21609819 [PubMed - indexed for MEDLINE] Free Article
- Kokovay E, Goderie S, Wang Y, Lotz S, Lin G, Sun Y, Roysam B, Shen Q, Temple S. Adult SVZ lineage cells home to and leave the vascular niche via differential responses to SDF1/CXCR4 signaling. Cell Stem Cell. 2010 Aug 6;7(2):163-73. doi: 10.1016/j.stem.2010.05.019.
- Neural Stem Cell Institute