Discovering

INDIVIDUAL RESEARCHER

Peter A. Vincent , Ph.D.
Professor
e-mail: vincenp@mail.amc.edu

Education

1988 - Ph.D. from Dept. of Physiology, Albany Medical College

Current Research

Endothelial cells line the inner walls of blood vessels where they play an active role in controlling many physiological functions of the vasculature. One such function of the endothelium is regulating the flux of proteins and fluid from the vascular space into the interstitium. Under normal conditions endothelial cells are tightly connected to one another by proteins on adjacent cells and there is very little flux of protein through the spaces between these cells. However, a number of pathological states, such as hypertension or inflammation, have been shown to be associated with increased flux of protein across the endothelium. Such as increases in the paracellular flux of protein have been implicated in the initiation of vascular diseases such atherosclerosis or vascular remodeling during systemic and/or pulmonary hypertension. Adherens junctions are a major type of cell-cell junction that contributes to the maintenance of endothelial monolayer integrity. Adherens junctions are formed by transmembrane proteins called cadherins that bind in a homophilic fashion to connect one endothelial cell to another. The cytoplasmic portion of cadherins are connected to the actin cytoskeleton by a complex network of proteins. The research in this lab focuses on how the cadherins and proteins in the adherens junction are involved in controlling the paracellular flux of proteins across the vascular endothelium. Transforming growth factor ß (TGFß) is a cytokine which has been shown to alter endothelial cell morphology resulting in an increased flux of proteins across the endothelial cell barrier. Current investigations are underway to determine the role of adherens junction disassembly in the signal transduction pathway that leads to TGFß induced decreases in endothelial monolayer integrity. Models are also being pursued to utilize molecular biology techniques in vitro using monolayers of isolated endothelial cells and in intact organs using isolated perfused rat lungs to determine the role of the adherens junction in the maintenance of endothelial monolayer integrity.

PubMed Publications

1. Chiasson CM, Wittich KB, Vincent PA, Faundez V, Kowalczyk AP. p120-catenin inhibits VE-cadherin internalization through a Rho-independent mechanism. Mol Biol Cell. 2009 Apr;20(7):1970-80.



2. Ferreri DM, Minnear FL, Yin T, Kowalczyk AP, Vincent PA. N-cadherin levels in endothelial cells are regulated by monolayer maturity and p120 availability. Cell Commun Adhes. 2008 Nov;15(4):333-49.



3. Alcaide P, Newton G, Auerbach S, Sehrawat S, Mayadas TN, Golan DE, Yacono P, Vincent P, Kowalczyk A, Luscinskas FW. p120-Catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylation. Blood. 2008 Oct 1;112(7):2770-9